Activation of nuclear factor-κB and aberrant gene expression of interleukin 6 in middle ear cholesteatoma
10.3760/cma.j.issn.1673-0860.2009.03.004
- VernacularTitle:中耳胆脂瘤组织中核因子κB的活化及白细胞介素6的异常表达
- Author:
Yu XU
1
;
Ze-Zhang TAO
;
Qing-Quan HUA
;
Xin-Chun WANG
;
Bo-Kui XIAO
;
Shi-Ming CHEN
Author Information
1. 武汉大学人民医院
- Keywords:
Cholesteatoma,middle ear;
NF-kappa B;
Interleukin-6
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(3):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the activation of NF-κB in middle ear cholesteatoma, discuss the relationship of NF-κB and the gene expression of IL-6 and explore the pathogenesis of middle ear cholesteatoma. Methods Ten cases of cholesteatoma and 6 cases of normal external meatal skin were obtained from middle ear surgery. The NF-κB DNA binding activity and the mRNA level of IL-6 in these two kinds of tissues were detected by electrophoretic motility shift assay (EMSA) and Reverse transcription polymerase chain reaction(RT-PCR) respectively. The relationship of NF-κB DNA binding activity and the mRNA level of IL-6 were analyzed. Results The NF-κB DNA binding activities of cholesteatoma [(15.9±8.2) %] were higher than those in normal skin [(1.36 ± 0.94) %, t = 3.502, P < 0.05]. The expression of IL-6 mRNA was increased significantly in patients with cholesteatoma, as compared with that in the control specimens (t = 2.166 ,P < 0.05) and had a significant positive correlation with NF-κB DNA binding activity (r = 0.752, P < 0.05). Conclusions The II.,-6 mRNA expression in cholesteatoma is closely related with the activity of NF-κB. It is tempting to speculate that NF-κB play a key role in the activation of cytokine in cholesteatoma.
