Tumor necrosis factor-alpha enhances the effect of endothelin on renal vasoconstriction in isolated perfused rat kidney.
- Author:
Ying WEN
1
;
Jing-yan WANG
;
Pei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Channels; metabolism; Endothelin-1; pharmacology; Heparin; pharmacology; Hepatorenal Syndrome; etiology; metabolism; In Vitro Techniques; Inositol 1,4,5-Trisphosphate Receptors; Kidney; blood supply; Male; Rats; Rats, Wistar; Receptors, Cytoplasmic and Nuclear; metabolism; Renal Artery; physiopathology; Tumor Necrosis Factor-alpha; analysis; pharmacology; Vasoconstriction; drug effects
- From: Chinese Journal of Hepatology 2003;11(10):583-585
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of tumor necrosis factor-alpha (TNF-alpha) in the pathogenesis of hepatorenal syndrome.
METHODSBy isolated perfused kidney technique, rat kidneys were perfused at a constant flow. Changes in perfusion pressure (mmHg) were consecutively measured with multi-functional physiology recorder. After TNF-alpha or heparin treated 90 minutes, the perfusion pressure stimulated by endothelin-1 (ET-1) was detected.
RESULTSTNF-alpha and heparin didn't modify the baseline perfusion pressure. When ET-1 was added at 2 nmol/L, the perfusion pressures increased to (47+/-9) mmHg, (97+/-36) mm Hg and (11+/-6) mm Hg in control, TNF-alpha and heparin (10mg/L) treated group, respectively, which were different among the three groups (t>or=3.811, P<0.01). No pathological damages were found in kidney tissues from all the groups after being stained with hematoxylin/eosin.
CONCLUSIONTNF-alpha plays an important role in the pathogenesis of hepatorenal syndrome by promoting renal vasoconstriction.
