Expression and location of heme oxygenase in the lung of experimental cirrhotic rats.
- Author:
Xiao-li ZHOU
1
;
De-an TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carbon Tetrachloride Poisoning; Heme Oxygenase (Decyclizing); analysis; biosynthesis; Heme Oxygenase-1; Liver Cirrhosis, Experimental; enzymology; Lung; enzymology; Male; Nitric Oxide; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Hepatology 2003;11(10):599-601
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo observe the function of heme oxygenase (HO) in the lung damage in hepatic cirrhosis rats.
METHODSLiver cirrhosis model rats were made by CCl4. Lung samples taken from normal and cirrhotic rats were examined for HO-1 and HO-2 protein and expression distribution with immunohistochemical staining and western blot.
RESULTSLiver cirrhosis model rats were successfully constructed. There was a notable increase of HO-1 staining (0.062+/-0.021 vs 0.185+/-0.044, t=11.24, P<0.01) and protein expression (0 vs 5294.92+/-46.02, t=11.45, P<0.01) in both vascular and bronchial smooth muscle cells and endothelium in cirrhotic rats, however, no statistical difference of HO-2 between cirrhotic and normal rats was observed.
CONCLUSIONThe HO-CO pathway is probably involved in the pathogenesis of lung damage in hepatic cirrhosis rats.
