Cloning and expressing the E2 subunit of pyruvate dehydrogenase complex.
- Author:
Jun ZHAO
1
;
Cui-li SHU
;
Li LEI
;
Jing LI
;
Rong GAO
;
Yun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Molecular; Dihydrolipoyllysine-Residue Acetyltransferase; Enzyme-Linked Immunosorbent Assay; Humans; Liver Cirrhosis, Biliary; blood; diagnosis; immunology; Lymphocytes; enzymology; Polymerase Chain Reaction; Pyruvate Dehydrogenase Complex; analysis; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2003;11(10):602-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo construct the expression vector of the pyruvate dehydrogenase complex E2 subunit gene (PDC-E2).
METHODSThe PDC-E2 gene was amplified from human lymphocytes with RT-PCR, and was cloned into pExSecI vector to induce the PDC-E2 expression. The products were identified with western blot and ELISA.
RESULTSThe expression vector pExSecI/PDC-E2 was successfully constructed. The products could be identified by the specific self-antibodies in the sera from the primary biliary cirrhosis patients.
CONCLUSIONHigh efficient expression vector of PDC-E2 lays the foundation for serum assay of primary biliary cirrhosis patients with prokaryotic expressing PDC-E2.
