Effects of Chinese herbal compound on the expression of hepatocyte cytochrome P450IIE1 in rats with alcoholic fatty liver.
- Author:
Ning DAI
1
;
Min-de ZENG
;
Yan-shen PENG
;
Ji-qiang LI
;
De-kai QIU
;
Lun-gen LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytochrome P-450 CYP2E1; metabolism; Drugs, Chinese Herbal; pharmacology; Fatty Liver, Alcoholic; pathology; Gene Expression; Hepatocytes; drug effects; enzymology; Immunohistochemistry; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Hepatology 2003;11(11):657-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Chinese herbal compound (CHC) on the expression of hepatocyte cytochrome P450IIE1 in rat model of alcoholic fatty liver (AFL).
METHODSThe AFL rats models were established by administering the drinking water with 40%(v/v) ethanol, and the changes of pathology in liver and hepatocyte P450IIE1 expression, as well as the contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), vitamin E (VitE) in liver were detected and compared with those in the control group.
RESULTSFatty degeneration in liver recovered normally in the CHC-treated group. Immunohistochemical and in situ hybridization examination showed that CHC could inhibit the hepatocyte cytochrome P450IIE1 expression markedly, and restore the contents of MDA, SOD, GSH, VitE to nearly normal range.
CONCLUSIONCHC can prevent AFL through inhibiting the hepatocyte cytochrome P450IIE1 expression markedly
