Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment.
- Author:
Yu-tao ZHAN
1
;
Lai WEI
;
Hong-song CHEN
;
Xu CONG
;
Ran FEI
;
Yu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; pathology; Cell Differentiation; physiology; Environment; Hematopoietic Stem Cells; pathology; Hepatocytes; pathology; Liver Cirrhosis; pathology; Male; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Hepatology 2003;11(11):673-675
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.
METHODSRat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.
RESULTSThe PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.
CONCLUSIONBone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts
