Inhibition of survivin expression in liver cancer cells by shRNA.
- Author:
Ge YAN
1
;
Ai-long HUANG
;
Ni TANG
;
Bing-qian ZHANG
;
Dan PU
;
Ming-que XIANH
;
Yin-hua LAN
;
Gang WU
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression Regulation, Neoplastic; Genetic Therapy; Humans; Inhibitor of Apoptosis Proteins; Liver Neoplasms; genetics; therapy; Microtubule-Associated Proteins; genetics; Neoplasm Proteins; RNA Interference; RNA, Small Interfering; genetics
- From: Chinese Journal of Hepatology 2003;11(12):712-715
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the plasmid containing short hairpin RNA (shRNA) of survivin in order to suppress the expression of survivin gene in HepG2 and SMMC-7721.
METHODSTwo 20 to 21 bp reverse repeated motifs of survivin target sequence with 4 bp or 8 bp spacer were synthesized respectively and inserted into plasmid pTZU6+1 to generate the plasmid pshRNA-survivin1 and pshRNA-survivin2; plasmid pEGFP-C1-survivin and pshRNA-survivin1 or pshRNA-survivin2 plasmid were cotransfected into liver cancer cell HepG2 and SMMC-7721 to detect effect of GFP expression respectively and analyze the inhibition of survivin gene.
RESULTSThe recombinant plasmid pshRNA-survivin1 and pshRNA-survivin2 were successfully constructed. The recombinant plasmids suppress the survivin expression by 80% in HepG2 and SMMC-7721.
CONCLUSIONThe result showed that the short hairpin RNA of survivin can efficiently suppress it's expression in HepG2 and SMMC-7721.
