Therapeutic effect of secretive endostatin eukaryotic expressing plasmid on mouse hepatoma.
- Author:
Pei-yuan LI
1
;
Ju-sheng LIN
;
Zuo-hua FENG
;
Hui ZHANG
;
He-jun ZHOU
;
Jin-yan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Endostatins; genetics; secretion; Genetic Therapy; Liver Neoplasms, Experimental; therapy; Male; Mice; Plasmids
- From: Chinese Journal of Hepatology 2003;11(12):716-718
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct and express secretive endostatin eukaryotic plasmid for treatment of hepatoma.
METHODSMouse Igk signal peptide sequence was synthesized and cloned into pcDNA3.1 with endostatin gene. The supernant of BHK-21 transfected with recombinant was used to culture ECV304. The proliferation of latter was evaluated by MTT assay. H22 was inoculated intramusclely, then naked DNA of endostatin plasmid was injected into the inoculation site. Tumors were dissected and weighted after treatments. All data was analyzed by SPSS10.0.
RESULTSThe supernant of BHK-21 transfected with recombinant can inhibit the proliferation of ECV304 by 29.2%. Tumor weight lighter after injected with naked pSecES (1.34 g+/-0.96g) compared with naked pcDNA3.1 (2.70g+/-0.82g) and saline (3.73g+/-1.41g).
CONCLUSIONThe endostatin eukaryotic plasmid was constructed and it can be used for gene therapy on hepatoma.
