Changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue.
- Author:
Guo-qian CHEN
1
;
Qing ZHANG
;
Yan-fang XU
;
Wan-zhong ZHANG
;
Ming GUAN
;
Bing SU
;
Hui-qi LIANG
;
Yuan LU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; chemistry; Carbohydrates; chemistry; Carcinoma, Hepatocellular; enzymology; pathology; Chromatography, Affinity; Humans; Lectins; metabolism; Liver Neoplasms; enzymology; pathology; Neoplasm Invasiveness
- From: Chinese Journal of Hepatology 2003;11(12):739-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of sugar chain structures of alkaline phosphatase (ALP) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (HCC).
METHODSThe binding ratios of ALP from 9 normal liver tissues, 16 hepatoma tissues and 16 noncancerous tissues surrounding hepatoma were analysed by affinity chromatography on various lectin columns including leukoagglutinating phytohemagglutinin (L-PHA), lentil lectin (LCA), Datura stramonium agglutinin (DSA), erythroagglutinating phytohemagglutinin (E-PHA) and Sambucus nigra bark agglutinin (SNA).
RESULTSThe binding ratios of ALP on L-PHA (22.94%+/-5.30%), DSA (55.97%+/-13.72%), LCA (38.16%+/-8.87%), E-PHA (11.56%+/-4.81%) and SNA (69.80%+/-13.71%) in HCC tissues were significantly increased (P<0.01) compared with that in normal liver tissues (L-PHA 5.89%+/-2.75%, DSA 36.20%+/-11.58%, LCA 17.90%+/-6.71%, E-PHA 5.38%+/-2.20%, SNA 57.32%+/-11.27%), respectively. t values between the two groups were 8.94, 3.64, 5.94, 3.62 and 2.32, respectively. L-PHA-binding ratio (25.84%+/-4.67%) of ALP in HCC with invasiveness was significantly higher than that (18.10%+/-3.64%) without invasiveness (t=3.71, P<0.01).
CONCLUSIONThe changes of ALP sugar chain structures occur in HCC tissue. b1-6 branching sugar chain structure of ALP is related to the invasiveness of HCC.
