Different effects of several signal pathway after liver transplantation.
- Author:
Guo-xun LI
1
;
Nian-qiao GONG
;
Qi-fa YE
;
Hui GUO
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Immunohistochemistry; In Situ Hybridization; Liver Transplantation; MAP Kinase Signaling System; Mitogen-Activated Protein Kinases; analysis; Signal Transduction; physiology; Tumor Suppressor Protein p53; analysis; ras Proteins; analysis
- From: Chinese Journal of Hepatology 2003;11(12):742-744
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo explorer the change of several signal pathway and their signal after liver transplantation.
METHODSClassified 34 punctured donor liver samples and 10 normal liver samples as A (no rejection) groups, B (mild/moderate acute rejection) groups, C (serious acute rejection) groups, D (chronic rejection/fibrosis) groups and E (control) groups, MAPK, Ras and p53 were performed immunohistochemistry analysis and image analysis. MAPK and Ras were performed in situ hybridizition. Then image analysis was performed.
RESULTSThe protein expression of MAPK, Ras, increase by turns of A, B and C groups (1.42+/-0.28, 3.88+/-0.87, 6.68+/-0.57 in MAPK; 1.27+/-0.12, 2.80+/-0.30, 3.93+/-0.20 in Ras; corresponding), and decrease by turns of D and E groups (1.49+/-0.37, 0.88+/-0.20 in MAPK; 1.47+/-0.21, 1.01+/-0.12 in Ras; corresponding, F=178.39 in MAPK and 320.59 in Ras, groups B, C vs groups A, D, E, P<0.001 in MAPK and Ras), The protein expression of p53 is higher in treated groups (The results of groups A to E are 2.09+/-0.13, 2.39+/-0.11, 2.03+/-0.19, 2.26+/-0.18 and 0.35+/-0.08, corresponding, F=360.08, groups E vs groups A, B, C, D, P<0.001). Expression of MAPK, Ras mRNA is as same as that of protein.
CONCLUSIONThe MAPKs pathway has role in rejection response after liver transplantation. And it seemed that the MAPKs and p53 are one regulation mechanism for protecting the hepatocyte from damage after liver transplantation.
