Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells.
- Author:
Xu LI
1
;
Ying MENG
;
Gao-su ZHOU
;
Zhen-shu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Humans; Kupffer Cells; cytology; NF-kappa B; metabolism; RNA Interference; RNA, Small Interfering; genetics; Receptor, Angiotensin, Type 1; genetics; metabolism
- From: Journal of Southern Medical University 2009;29(1):20-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.
METHODSThe expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).
RESULTSAT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.
CONCLUSIONAng II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor.