Expression of Aurora-B in human glioma tissue and its significance.
- Author:
Li-bo SUN
1
;
Shuang-lin FU
;
Yi-nan LUO
;
Huan CHEN
;
Wen-chen LI
;
Peng-fei GE
Author Information
- Publication Type:Journal Article
- MeSH: Aurora Kinase B; Aurora Kinases; Biomarkers, Tumor; metabolism; Brain Neoplasms; enzymology; pathology; Female; Glioma; metabolism; pathology; Humans; Male; Protein-Serine-Threonine Kinases; genetics; metabolism; RNA, Messenger; genetics; metabolism; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2009;29(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of Aurora-B in human glioma tissue and its significance.
METHODSThe total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.
RESULTSAurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.
CONCLUSIONAurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.
