Epithelial sodium channel subunit mRNA expression in acutely isolated rat alveolar type II cells.
- Author:
Wang-mei ZHOU
1
;
Lin HE
;
Tao-ping LI
;
Jie YOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Epithelial Sodium Channels; genetics; metabolism; Male; Pulmonary Alveoli; cytology; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2009;29(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the epithelial sodium channel (ENaC) subunit mRNA expression in acutely isolated rat alveolar type II (ATII) cells.
METHODSAcutely isolated ATII cells from 20 SD rats were purified and ENaC alpha, beta, gamma-subunit mRNA levels were determined by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSmRNA expressions of all the subunits were detected in the ATII cells, and ENaC alpha-subunit mRNA showed significantly higher expression than beta- and gamma-subunit mRNAs, and the expressions of the latter two mRNAs were comparable.
CONCLUSIONAs the predominant ENaC subunit expressed at the mRNA level in rat ATII cells, the alpha-subunit of ENAC plays an important role in alveolar fluid clearance.
