Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis.
- Author:
Xiao-xia WANG
1
;
Xiao-bao YAO
;
Xian-sheng JI
;
Hong-liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; Caspase 3; biosynthesis; genetics; Genetic Vectors; Humans; Nasopharyngeal Neoplasms; genetics; pathology; Recombinant Proteins; biosynthesis; genetics; Transfection; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2009;29(1):75-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells (CNE2).
METHODSRecombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique. CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction. The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.
RESULTSTransfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.
CONCLUSIONThe recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.
