Effect of intense pulsed light on transforming growth factor-beta1 mRNA expression in rat skin.
- Author:
Ming-li WANG
1
;
Da-lie LIU
;
Qiang YUAN
;
Ben-jun DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Male; Phototherapy; adverse effects; methods; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Rejuvenation; Skin; metabolism; radiation effects; Transforming Growth Factor beta1; genetics; metabolism; radiation effects
- From: Journal of Southern Medical University 2009;29(1):92-96
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of intense pulsed light (IPL) on transforming growth factor-beta1 mRNA (TGF-beta1 mRNA) expression in rat skin and explore the molecular mechanisms of photorejuvenation.
METHODSFifteen SD rats were exposed to IPL in 3 dermal regions with triple pulses (duration of 4, 5, and 6 ms) at the energy density of 34 J/cm2 and pulse delay of 20 or 25 ms. On days 1, 3, 5, 7, 15, and 30 after the treatment, skin specimens from the treated and non-treated areas were obtained to detect TGF-beta1 mRNA expression with in situ hybridization.
RESULTSIn the UPL-exposed skin areas, TGF-beta1 mRNA expression was detected in the epidermal keratinocytes and dermal cells 1 day after the exposure, reaching the highest expression level on day 7 followed by gradual decrement since day 15, and till day 30, only weak expression was found in the dermal cells. In the non-exposed regions, the cells remained negative for TGF-beta1 mRNA.
CONCLUSIONIPL can enhance TGF-beta1 mRNA expression in the skin, suggesting that TGF-beta1 plays an important role in dermal remodeling in photorejuvenation.
