MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line.
- Author:
Fang-xuan HAN
1
;
Hui LIN
;
Ling RU
Author Information
- Publication Type:Journal Article
- MeSH: Antimetabolites, Antineoplastic; pharmacology; Breast Neoplasms; pathology; Cell Line, Tumor; Colorimetry; methods; Coloring Agents; Drug Screening Assays, Antitumor; Female; Fluorouracil; pharmacology; Humans; Tetrazolium Salts
- From: Journal of Southern Medical University 2009;29(1):97-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).
METHODSThe chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.
RESULTS5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.
CONCLUSIONSMTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
