Effects of all-trans retinioic acid and tazarotene on MMP-1 and TIMP-1 expression in cultured human fibroblasts after heat shock.
- Author:
Ping LIU
1
;
Sheng-shun TAN
;
Qiong-yu WANG
;
Yong-xian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Fibroblasts; cytology; metabolism; Heat-Shock Response; Humans; Matrix Metalloproteinase 1; genetics; metabolism; Nicotinic Acids; pharmacology; Skin Aging; radiation effects; Tissue Inhibitor of Metalloproteinase-1; genetics; metabolism; Tretinoin; pharmacology
- From: Journal of Southern Medical University 2009;29(2):217-219
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular mechanism of dermal damage in heat shock-induced skin aging by observing the expressions of metalloproteinase-1 (MMP-1) and tissue inhibitor of MMP-1 (TIMP-1) in retinoic acid-treated cultured human fibroblasts with heat shock.
METHODSCultured human fibroblasts were treated with tazarotene or all-trans-retinioic acid (at-RA) after heat shock for 30 min in 43 degrees celsius; water bath. Twenty-four hours later, MMP-1 and TIMP-1 contents in the supernatant of the cell culture medium were measured using enzyme-linked immunosorbent assay (ELISA).
RESULTSBoth tazarotene and at-RA dose-dependently reduced the expression of MMP-1 and increased the expression of TIMP-1 in cultured human fibroblasts exposed to heat shock, and tazarotene produced stronger effect than at-RA.
CONCLUSIONRetinoic acid can reduce the expression of MMP-1 and increase the expression of TIMP-1 in cultured human fibroblasts, suggesting its therapeutic potential for heat shock-induced skin aging.
