Expression of CD45 in the serum of patients with Crohn's disease.
- Author:
Liang KANG
1
;
Zu-li YANG
;
Lei WANG
;
Mei-jin HUANG
;
Wei LIU
;
Ming-tao LI
;
Jian-ping WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Case-Control Studies; Crohn Disease; blood; immunology; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Leukocyte Common Antigens; blood; Male; Molecular Sequence Data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Journal of Southern Medical University 2009;29(2):259-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for differentially expressed proteins in the serum of patients with Crohn's disease.
METHODSSerum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.
RESULTS2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.
CONCLUSIONCD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.
