Effects of recombinant human erythropoietin on hypoxia inducible factor-1alpha expression in the retina of rabbits with acute high intraocular pressure.
- Author:
Jian-ming WANG
1
;
Yan-ping SONG
;
Nai-xue SUN
;
Na HUI
;
Shi-ping ZHAO
;
Kai HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Down-Regulation; Erythropoietin; pharmacology; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Neuroprotective Agents; pharmacology; Ocular Hypertension; metabolism; Rabbits; Recombinant Proteins; Reperfusion Injury; prevention & control; Retina; metabolism; Retinal Vessels; metabolism
- From: Journal of Southern Medical University 2009;29(2):271-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of recombinant human erythropoietin (rhEPO) on the expression of hypoxia inducible factor-1alpha (HIF-1alpha) in the retina of rabbits with acute high intraocular pressure and investigate the mechanism of rhEPO in protecting the retina from ischemia-reperfusion injury.
METHODSAcute high intraocular pressure was induced in the rabbits by perfusion of normal saline into the anterior chamber, and rhEPO was injected subcutaneously. The changes in HIF-1alpha protein expression in the retina was observed by immunohistochemistry on days 1, 3, 7, and 14 after retinal ischemia- reperfusion.
RESULTSHIF-1alpha expression was not observed in the retina of the normal control rats, but intense HIF-1alpha expression was found in the model group (P<0.01). In rabbits with rhEPO injection and those in the model group, the patterns of HIF-1alpha expression alterations were similar, but the HIF-1alpha-positive cells in the retina were significantly fewer in rhEPO group (P<0.05).
CONCLUSIONrhEPO can down-regulate HIF-1alpha expression in the retina of rabbits with acute high intraocular pressure, which may be one of the mechanisms that rhEPO protects the retina from ischemia-reperfusion injury.
