Single nucleotide polymorphisms of DAT and DRD(2) genes in Han Chinese population and their association with stuttering.
- Author:
Chun-Hui PAN
1
;
Lu-Ping SONG
;
Jie DU
;
Jie LAN
;
Chun-Mei WU
;
Li-Juan WU
;
Lan LIN
;
Wei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Asian Continental Ancestry Group; genetics; Case-Control Studies; China; ethnology; Dopamine Plasma Membrane Transport Proteins; genetics; Female; Genotype; Humans; Male; Polymorphism, Single Nucleotide; Receptors, Dopamine D2; genetics; Stuttering; genetics; Young Adult
- From: Journal of Southern Medical University 2009;29(3):375-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the correlations of dopamine transporter gene (DAT) and dopamine D(2) receptor gene (DRD2) to stuttering.
METHODSTo examine the correlations of the 5 single nucleotide polymorphisms (SNPs) in dopaminergic gene (C252T, C1804T, and C1820T in DAT gene, and T1054C and C1072T in DRD(2) gene) to stuttering in Han Chinese individuals, a case-control study involving 112 patients with stuttering and 112 gender-matched controls was carried out. Genotyping was performed by a combined approach using polymerase chain reaction (PCR) and pyrosequencing.
RESULTSC1804T showed no polymorphism in either the patients or the control subjects and was therefore excluded from the following analysis. The C allele frequency at C1072T site was significantly higher, but T allele frequency significantly lower in the stuttering group than in the control group. The patients had significantly higher CC and lower CT genotype frequencies than the control group. There were no significant differences in the allelic frequencies of C252T, C1820T and T1054C between the patients and the controls, suggesting a Hardy-Weinberg equilibrium at these 3 loci.
CONCLUSIONThe presence of the C allele at C1072T in DRD(2) gene is associated with increased susceptibility to stuttering in Han Chinese, whereas the T allele provides protection against the onset of stuttering.
