Apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field.
- Author:
Qiu TAN
1
;
Wen-jun SUN
;
Yong-miao PAN
;
Hui-lan SUN
;
Gen-lin HU
;
Huai JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Caspase 3; metabolism; Cells, Cultured; Female; Gene Expression Regulation; Humans; Magnetic Fields; adverse effects; Pregnancy; Proto-Oncogene Proteins c-bcl-2; metabolism; Trophoblasts; metabolism; Tumor Suppressor Protein p53; metabolism; bcl-2-Associated X Protein; metabolism; fas Receptor; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):581-585
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field and to investigate the possible mechanism of human reproductive health effects caused by 50 Hz magnetic field.
METHODSCultured human villous trophoblasts were exposed to 50 Hz magnetic field at 0.4 mT for 6, 48, 72 hours. Gene expressions of Bcl-2, Bax, Caspase-3, p53 and Fas were analyzed using real-time reverse transcription polymerase chain reaction (RT-PCR) assay.
RESULTSWithin 72 hours, the average fold change for each gene was near 1.00, and there was no significant difference on expression pattern in each gene between exposure and control groups (P > 0.05).
CONCLUSION0.4 mT 50 Hz magnetic field does not affect the apoptosis-related gene expression of human villous trophoblasts in vitro.
