Construction of anti-lung tumor gene differentially expressed bank of wild mouse.
- Author:
Feng-shan WANG
1
;
Ying-tang GAO
;
Jiang-shan YANG
;
Tong GUAN
;
Rei-dong MU
;
Zhi-li QI
;
Guo-de YU
;
Li JING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA, Complementary; genetics; Female; Gene Expression Profiling; Gene Library; Lung Neoplasms; genetics; Male; Mice; Mice, Inbred Strains; genetics; Oligonucleotide Array Sequence Analysis; RNA, Messenger; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):594-599
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the anti-lung tumor gene differentially expressed bank of wild mouse and to explore the mechanisms of the TW wild mouse suppressing the occurring of lung tumor.
METHODSUsing suppression subtractive hybridization (SSH) technique, the differentially expressed genes between TAF1 mouse and A/wy mouse were selected out and the subtracted cDNA bank was constructed. 166 clones were performed DNA sequencing and then were assayed by blast programme.
RESULTSAmong the blast results of 166 differentially expressed clones, 87 known genes (mRNA or cDNA) were in homology with 134 clones and were divided into 7 classifications according to the biological role.14 DNA fragments were in homology with 32 clones, in which 20 clones were in homology with 9 mouse DNA sequences, 2 clones were in homology with one bacterial gene sequences and 3 clones were clone vector.
CONCLUSIONWith SSH technique, the anti-lung tumor gene differentially expressed bank of wild mouse are successfully constructed.
