Protective effect of NEP on Abeta-induced apoptosis in PC12 cells.
- Author:
Ji-Ping ZENG
1
;
Ling-Ling YANG
;
Wei-Fang WU
;
Feng KONG
;
Xiao-Yan HU
;
Xing CUI
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; pharmacology; Animals; Apoptosis; drug effects; Caspase 3; metabolism; Endopeptidases; genetics; metabolism; PC12 Cells; Rats; Transfection
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(12):722-725
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the effect of NEP on Abeta -induced apoptosis in PC12 cells.
METHODSPC12 cells that stably express NEP is generated and the effect of NEP on the process of apoptosis induced by Abeta is analyzed, including the viability of the cells, the production of LDH, ROS and ATP, the activity of Caspase-3.
RESULTSNEP could improve the viability of cells and the production of ATP, inhibit the release of LDH and ROS. In the same time, the activity of caspase-3 descended (P < 0.05). But iNEP had not significant effect on cells apoptosis (P > 0.05).
CONCLUSIONNEP has the protective effect on Abeta-induced apoptosis in PC12 cells.
