Role of activator protein-1 in pathogenesis of silicosis: an in-vitro study.
- Author:
Xiang LI
1
;
Yong-bin HU
;
Hai-ying JIANG
;
Jin-sheng WANG
;
Ji-fang WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Curcumin; pharmacology; Macrophages; drug effects; metabolism; Mice; Proto-Oncogene Proteins c-fos; metabolism; Proto-Oncogene Proteins c-jun; metabolism; RNA, Messenger; genetics; Silicon Dioxide; pharmacology; Transcription Factor AP-1; antagonists & inhibitors; metabolism; Transforming Growth Factor beta1; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of activator protein-1 (AP-1) in the up-regulation expression of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1(TGF-beta(1)) in silica-stimulated macrophage cells (RAW264.7).
METHODSRAW264.7 cells were treated with AP-1 inhibitor Curcumin. The expression of c-jun and c-fos in nuclear protein was detected by western blotting. The level of TNF-alpha and TGF-beta(1) protein in the cell supernatant was measured using enzyme-linked immunoadsorbent assay (ELISA). Meanwhile the expression of TNF-alpha and TGF-beta(1) mRNA was also monitored by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSThe nucleoprotein expression of c-jun and c-fos in 10 and 20 micromol/L Curcumin prevention group (1.150 +/- 0.020, 1.010 +/- 0.108, 80.430 +/- 0.023, 0.256 +/- 0.015) were lower than those in silica-stimulated group (1.550 +/- 0.029, 0.860 +/- 0.036) (P < 0.01). In 20 micromol/L Curcumin prevention group and silica stimulated group, the expression of TNF-alpha protein were 23.58 +/- 45.78 and 32.12 +/- 5.34, and the expression of TGF-beta(1) protein were 1582.18 +/- 437.52 and 55.60 +/- 5.51 (P < 0.05 =; the expression of TNF-alpha, TGF-beta(1) mRNA were 0.74 +/- 0.01, 0.22 +/- 0.04 and 2.27 +/- 0.33, 2.96 +/- 0.15 (P < 0.05 =.
CONCLUSIONThe expression of TNF-alpha, TGF-beta(1) mRNA and proteins is associated with activation of AP-1 in silica-stimulated macrophage cells.
