PretreatedExtract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area.

Joon Ha Park; Tae-kyeong Lee; Bing-chun Yan; Bich-na Shin; Ji Hyeon Ahn; In Hye Kim; Jeong Hwi Cho; Jae-chul Lee; In Koo Hwang; Jong Dai Kim; Seongkweon Hong; Young Joo Lee; Moo-ho Won; Il Jun Kang

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PretreatedExtract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area.

Author: Joon Ha PARK ¹ ; Tae-Kyeong LEE ² ; Bing-Chun YAN ³ ; Bich-Na SHIN ⁴ ; Ji Hyeon AHN ¹ ; In Hye KIM ² ; Jeong Hwi CHO ² ; Jae-Chul LEE ² ; In Koo HWANG ⁵ ; Jong Dai KIM ⁶ ; Seongkweon HONG ⁷ ; Young Joo LEE ⁸ ; Moo-Ho WON ² ; Il Jun KANG ⁹
Author Information

1. Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Korea.
2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
3. Department of Traditional Chinese and Western Medicine, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu 225001, China.
4. Department of Physiology, College of Medicine, Hallym University, Chuncheon 24252, Korea.
5. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
6. Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, 24341, Korea.
7. Department of Surgery, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
8. Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul 04401, Korea.
9. Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Korea.
Publication Type:Journal Article
From: Chinese Medical Journal 2017;130(15):1796-1803
CountryChina
Language:English
Abstract:
BACKGROUNDGlehnia littoralis, as a traditional herbal medicine to heal various health ailments in East Asia, displays various therapeutic properties including antioxidant effects. However, neuroprotective effects of G. littoralis against cerebral ischemic insults have not yet been addressed. Therefore, in this study, we first examined its neuroprotective effects in the hippocampus using a gerbil model of transient global cerebral ischemia (TGCI).
METHODSGerbils were subjected to TGCI for 5 min. G. littoralis extract (GLE; 100 and 200 mg/kg) was administrated orally once daily for 7 days before ischemic surgery. Neuroprotection was examined by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. Gliosis was observed by immunohistochemistry for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1. For neuroprotective mechanisms, immunohistochemistry for superoxide dismutase (SOD) 1 and brain-derived neurotrophic factor (BDNF) was done.
RESULTSPretreatment with 200 mg/kg of GLE protected pyramidal neurons in the cornu ammonis 1 (CA1) area from ischemic insult area (F = 29.770, P < 0.05) and significantly inhibited activations of astrocytes (F = 22.959, P < 0.05) and microglia (F = 44.135, P < 0.05) in the ischemic CA1 area. In addition, pretreatment with GLE significantly increased expressions of SOD1 (F = 28.561, P < 0.05) and BDNF (F = 55.298, P < 0.05) in CA1 pyramidal neurons of the sham- and ischemia-operated groups.
CONCLUSIONSOur findings indicate that pretreatment with GLE can protect neurons from ischemic insults, and we suggest that its neuroprotective mechanism may be closely associated with increases of SOD1 and BDNF expressions as well as attenuation of glial activation.