EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata.
- Author:
Xingkui TAO
1
;
Aimin ZHANG
;
Jianping XUE
;
Jingtong TENG
;
Hedong LU
;
Tao XUE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Survival; drug effects; genetics; Dose-Response Relationship, Drug; Ethyl Methanesulfonate; pharmacology; Mutagenesis; drug effects; Mutation; drug effects; Pinellia; cytology; drug effects; genetics; physiology; Suspensions; Temperature; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(12):1731-1733
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata.
METHODUnder four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized.
RESULT AND CONCLUSIONThe results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.
