Protective effect of atractylenolide I on immunological liver injury.
- Author:
Changhe WANG
1
;
Qingguang GENG
;
Yuxuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chemical and Drug Induced Liver Injury; immunology; metabolism; pathology; prevention & control; Lactones; pharmacology; Lipopolysaccharides; adverse effects; Liver; drug effects; enzymology; metabolism; pathology; Male; Mice; Mycobacterium bovis; immunology; Oxidative Stress; drug effects; immunology; Sesquiterpenes; pharmacology
- From: China Journal of Chinese Materia Medica 2012;37(12):1809-1813
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of atractylenolide I on immunological liver injury induced by BCG and LPS.
METHODKunming mice were randomly divided into 6 groups: the normal group, the model group, positive control biphenyl group, the atractylenolide I high does group, the atractylenolide I middle dose group and the atractylenolide I low dose group (60, 120, 240 mg x kg(-1)), with 12 mice in each group. Immunological liver injury in mice was induced by BCG and LPS to compared liver index and spleen index and detect content of serum ALT, AST, MDA and GSH-px in serum and NO, iNOS, TNF-alpha in serum and liver homogenate. Liver pathological changes were observed by HE staining.
RESULTBoth of atractylenolide I and biphenyl remarkably decrease the increased live index and spleen index (P < 0.05), improve the histopathological changes in liver and pathological grades of liver tissues and relieve the inflammatory reaction induced by BCG and LPS. They showed a notable effect in improving MDA and GSH-px in serum.
CONCLUSIONAtractylenolide I can obviously protect immunological injury liver a dose-dependent manner within the range of test doses. Its mechanism may be related to release or over expression of inhibitory inflammatory medium such as NO, iNOS and TNF-alpha.
