Effect of curcumin on synapse-related protein expression of APP/PS1 double transgenic mice.
- Author:
Peng WEI
1
;
Ruisheng LI
;
Hong WANG
;
Ying REN
;
Haiyun SUN
;
Jinduo YANG
;
Pengwen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Protein Precursor; genetics; Animals; CA1 Region, Hippocampal; drug effects; metabolism; Curcumin; pharmacology; Disks Large Homolog 4 Protein; Gene Expression Regulation; drug effects; Guanylate Kinases; metabolism; Membrane Proteins; metabolism; Mice; Mice, Transgenic; Nerve Tissue Proteins; metabolism; Presenilin-1; genetics; Synapses; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(12):1818-1821
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of curcumin on the expression of synapse-related proteins PSD-95 and Shank1 in APP/PS1 double transgenic mice.
METHODThree-month-old APP/PS1 dtg mice were randomly divided into the model group, the positive Rosiglitazone control group and curcumin high (400 mg x kg(-1) x d(-1)), medium (200 mg x kg(-1) x d(-1)) and low (100 mg x kg(-1) x d(-1)) dose groups, with non-genetically modified mice with the same background as the normal group. After the oral administration for three months, immunohistochemistry and Western blot were adopted for detection.
RESULTAccording to the behavioral detection, the treatment group and the model group showed differences in the place navigation test and the spatial probe test to varying degrees (P < 0.01 or P < 0.05). The expression of PSD-95 and Shank1-positive cells of hippocampus CA1 region significantly decreased in model mice compared with normal control group (P < 0.01); while the curcumin intervention group showed recovery to some extend. Western blot results showed that the strap of PSD-95 protein expression became significantly thinner and lighter in the model group compared with the normal control group (P < 0.01); while the curcumin intervention group showed notably thicker and darker straps of PSD-95 protein expression (P < 0.05).
CONCLUSIONCurcumin can increase the expression of synapse-related proteins PSD95 and Shank1 in APP/PS1 double transgenic mice, improve structure and plasticity of synapse in APP/PS1 double transgenic mice and enhance their learning and memory abilities.
