Effect and mechanism of curcumol on angiogenesis activity of zebrafishes.
- Author:
Lili TIAN
1
;
Jianyong DONG
;
Changjiang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Embryo, Nonmammalian; blood supply; drug effects; metabolism; Gene Expression Regulation; drug effects; Neovascularization, Physiologic; drug effects; Sesquiterpenes; pharmacology; Vascular Endothelial Growth Factor A; genetics; Vascular Endothelial Growth Factor Receptor-2; genetics; Zebrafish; embryology; genetics; physiology
- From: China Journal of Chinese Materia Medica 2012;37(12):1822-1825
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo conduct a preliminary study on the effect of curcumol in promoting angiogenesis activity and its mechanism in zebrafishes, in order to provide basis for clinical prescription.
METHODZebrafishes biological model was established to, observe curcumol's effect on embryo blood vessel growth, blood vessel regeneration of adult fishes after tail-cutting and tissue regeneration of fish fries after tail-cutting. The relative fluorescence quantitative PCR method was adopted to determine the gene expression of vascular endothelial growth factor (VEGFA) and receptor VEGFR2 of fish fries after tail-cutting.
RESULTCurcumol contributed to angiogenesis of intersegmental blood vessels in zebrafishes embryos and speed up regeneration of blood vessels in adult fishes after tail-cutting. Furthermore, curcumol can increase the gene expression of VEGFA and VEGFR2 in fish fries.
CONCLUSIONCurcumol can promote angiogenesis in zebrafishes, and enhance the gene expression of VEGFA and VEGFR2 in fish fries after tail-cutting and speed up the regeneration of their tails.
