Protective effect of ligustrazine hydrochloride on homocysteine-injured ECV304 cells.
- Author:
Dongming XU
1
;
Dan WU
Author Information
- Publication Type:Journal Article
- MeSH: Cytoprotection; drug effects; Homocysteine; adverse effects; Human Umbilical Vein Endothelial Cells; cytology; drug effects; metabolism; Humans; Nitric Oxide; biosynthesis; Nitric Oxide Synthase; biosynthesis; Pyrazines; pharmacology
- From: China Journal of Chinese Materia Medica 2012;37(12):1836-1839
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the protective effect of ligustrazine hydrochloride on homocysteine-injured ECV304 cells.
METHODIn the in vitro study, human umbilical vein endothelial cells were selected as objects, with homocysteine as the molding agent, to judge the injury degree by monitoring NOS and NO contents. Based on that, the best homocysteine concentration in ECV304 cells, the best reaction time could be determined, and an endothelial cell injury model was established. After adding ligustrazine hydrochloride, NOS and NO contents in injured endothelial cells were determined to observe the protective effect of ligustrazine hydrochloride.
RESULTIt was proved that the optimal concentration of homocysteine on injured ECV304 cell was 1 mmol x L(-1), the best reaction time was 48 h. An injured endothelial cell model was established. At the same time, positive drug nitroglycerin and ligustrazine hydrochloride displayed a protection effect on injured ECV304 cells, NOS and NO formation were significantly increased compared with the model group.
CONCLUSIONLigustrazine hydrochloride has a protective effect on homocysteine-injured ECV304 cells. The model established in this study can be used to screen anti-myocardial ischemia drugs targeting at an endothelial cell protective agent.
