Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars.

Author: Daxia CHEN ¹ ; Xue ZHANG ; Longyun LI
Author Information

1. Chongqing Engineering Research Center of Lonicea Flos, Chongqing Research Center of Techniques and Engineering for Chinese Materia Medica Fine Variety Breeding and Evaluation, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. 17837@163.com
Publication Type:Journal Article
MeSH: Genetic Variation; Lonicera; genetics; Polymorphism, Genetic; Software
From: China Journal of Chinese Materia Medica 2012;37(13):1912-1916
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study genetic diversity and genetic relationships among Lonicera macranthoides cultivars.
METHODFive cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system diagram of genetic relationship was built by UPGMA.
RESULTTwenty ISSR primers amplified 186 bands with 103 (54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347(55.46%) polymorphic bands. Genetic distance ranges were 0.058 4-0.230 8 (by ISSRs) and 0.1071-0.2611 (by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic diversity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same.
CONCLUSIONThe genetic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.