Study on water-soluble constituents of Fritillaria unibracteata by HPLC fingerprints.
- Author:
Baozhong DUAN
1
;
Shilin CHEN
;
Dahua WEI
;
Linfang HUANG
;
Zhen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Dermatoglyphics; Drugs, Chinese Herbal; chemistry; Fritillaria; chemistry; Medicine, Chinese Traditional; Solubility; Water; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(13):1990-1993
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC fingerprint of water-soluble constituents of Fritillaria unibracteata.
METHODZorbax SB Aq C18 chromatographic column (4. 6 mm x 250 mm, 5 microm) was adopted for gradient elute with the mobile phase consisting of methanol and water. The flow rate was 1.0 mL x min(-1); the detection wavelength was 260 nm, and the temperature of sample manager was set at 25 degrees C. Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine (version 2.0) published by the State Pharmacopeia Committee of China was adopted for the fingerprint analysis on the 11 batches of F. unibracteata herbs.
RESULTThe 11 batches of F. unibracteata herbs had 14 common peaks, nine of which were identified with good separating degrees. The similarities of the 11 batches were more than 0. 970, with good quality homogeneity.
CONCLUSIONThe method is so accurate, highly reproducible and stable that it is suitable for the comprehensive quality evaluation of F. unibracteata herbs.
