Study on absorption ingredients of yuanhuzhitong pill by everted intestinal sac method.
- Author:
Xiaomeng CHEN
1
;
Yingchun ZHANG
;
Shuo LIN
;
Hongjun YANG
;
Hongwei WU
;
Shaojing LI
;
Defeng LI
;
Haiyu XU
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Animals; Chromatography, High Pressure Liquid; methods; Corydalis; chemistry; Drugs, Chinese Herbal; chemistry; pharmacokinetics; Intestinal Absorption; Intestines; metabolism; Male; Medicine, Chinese Traditional; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2012;37(13):2005-2011
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an everted intestinal sac method for determining absorption ingredients of yuanhuzhitong pill and study absorption characteristics of major chemical constituents of yuanhuzhitong pill.
METHODThe everted intestinal sac model was adopted. Intestinal sac fluid samples at different time points after administration of three concentrations of Yuanhuzhitong pill were collected and determined by HPLC. The accumulative absorbed doses of active constituents were calculated, while the proportion between samples of yuanhuzhitong pill and absorption ingredients was compared.
RESULTEight ingredients of Yuanhuzhitong pill can be detected in intestinal sac, they are protopine, palmatine, coptisine, imperatorin, berberine, byakangelicin, alpha-allocryptopine and tetrahydropalmatine. The absorption rate constants (Ka) of eight constituents increased in jejunum and ileum with the increase in concentration of Yuanhuzhitong pill extracts (P < 0. 05), indicating a passive absorption.
CONCLUSIONAs ingredients are selectively absorbed in intestinal sac, the everted intestinal sac method is selected to assess the intestinal absorption characteristics of ingredients of Yuanhuzhitong prescription.
