In vitro embryo culture of Epimedium wushanense.
- Author:
Haiqin ZHOU
1
;
Guosheng ZHIU
;
Qiaosheng GUO
;
Zuoyi LIU
;
Ning ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; Epimedium; embryology; Regeneration; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2012;37(14):2046-2051
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture.
METHODCullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA.
RESULTThe optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg x L(-1), IBA 2 mg x L(-1) and NAA 0.5 mg x L(-1) and the MS medium supplemented with IBA 2 mg x L(-1) and 6-BA 0.5 mg x L(-1), respectively. The optimum medium for callus differentiation was MS + 6-BA 1 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 1 mg x L(-1), and MS +6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) for shoots proliferation.
CONCLUSIONUsing embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.