Non-anthraquinones constituents from the roots of Knoxia valerianoides.
- Author:
Feng ZHAO
1
;
Sujuan WANG
;
Xiuli WU
;
Yang YU
;
Zhenggang YUE
;
Bo LIU
;
Sheng LIN
;
Chenggen ZHU
;
Yongchun YANG
;
Jiangong SHI
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medico, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line, Tumor;
Chromatography, High Pressure Liquid;
Humans;
Lignans;
analysis;
chemistry;
isolation & purification;
Mice;
Plant Roots;
chemistry;
Rubiaceae;
chemistry;
Sitosterols;
chemistry;
isolation & purification;
pharmacology;
Triterpenes;
chemistry;
isolation & purification;
pharmacology
- From:
China Journal of Chinese Materia Medica
2012;37(14):2092-2099
- CountryChina
- Language:Chinese
-
Abstract:
Twenty-one non-anthraquinones constituents were isolated for the first time from an ethanol extract of the roots of Knoxia valerianoides by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were identified by their physical-chemical properties and spectroscopic analysis including NMR and MS. The compounds include ten triterpenoids: ursolic acid (1), oleanolic acid (2), 2-oxo pomolic acid (3), pomolic acid (4), maslinic acid (5), rotungenic acid (6), tormentic accid (7), rotundic acid 3,23-acetonide (8), arjungenin (9), and 2alpha, 3beta, 19alpha, 23-tetrahydroxy-urs-12-en-28-oic acid (10), four sitosterones: (24R)-24-ethylcholesta-4,22-dien-3-one (11), 3-oxo-4-en-sitosterone (12), 7-oxostigmasterol (13), and 7-oxo-beta-sitosterol (14), two lignans: eudesmin (15) and ciwujiatone (16), one coumarin: cnidilin (17), and four simple aromatic analogues: 5-hydroxymethylenefural (18), 3-hydroxy-4-methoxybenzoic acid (19), benzoic acid (20), and 2-hydroxy-5-methxoycinnamaldehydes (21). In the in vitro assays against human cancer cell lines (HCT-8, Bel7402, BGC-823, A549, and A2780), against deserum and glutamate induced PC12-syn cell damage, and against HIV-1 replication, and inhibiting protein tyrosine phosphatase 1B (PTP1 B), LPS induced NO production in macrophage, and Fe(2+)-cystine induced rat liver microsomal lipid peroxidation, at a concentration of 1 x 10(-5) mol x L(-1), no compound showed activity.
