Effect and mechanism of gastrodin in relaxing isolated thoracic aorta rings in rats.
- Author:
Yingqiao ZHANG
1
;
Tao YU
;
Jiyang XU
;
Xiaohong BIAN
;
Jianliang XU
;
Yamei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; drug effects; physiology; Benzyl Alcohols; pharmacology; Calcium; metabolism; Endothelium, Vascular; physiology; Glucosides; pharmacology; In Vitro Techniques; Male; Norepinephrine; pharmacology; Rats; Rats, Sprague-Dawley; Vasodilation; drug effects
- From: China Journal of Chinese Materia Medica 2012;37(14):2135-2138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of gastrodin in relaxing isolated thoracic aorta rings in rats and discuss its possible mechanism.
METHODIsotonic tension of isolated thoracic aortic rings in rats with norepineprine (NE) and KCl was recorded to observe the vasodilatory effect of gastrodin and the influence of various drugs on it.
RESULTGastrodin had the effect in relaxing thoracic aortas with or without endothelium, and there was no significant difference. NG-nitro-L-argininemethylester (L-NAME, 1 x 10(-4) mol x L(-1)), methylene blue (MB, 1 x 10(-5) mol x L(-1)), indomethacin (INDO, 1 x 10(-5) mol x L(-1)) had no effect on the vasodilation action of gastrodin on thoracic aortas precontracted by NE. 4-aminopyrimide (4-AP, 1 x 10(-4) mol x L(-1)), tetrathylamonium (TEA, 1 x 10(-3) mol x L(-1)), BaCl2 (1 x 10(-4) mol x L(-1)) and glibenclamide (Gli, 1 x 10(-5) mol x L(-1)) could inhibit gastrodin's effect in relaxing thoracic aorta rings. In the absence of Ca2+, pre-incubated gastrodin showed a notable inhibitory effect in relaxing NE contraction.
CONCLUSIONGastrodin shows a dose-dependent and endothelium-independent effect in relaxing rat isolated thoracic aorta rings. The mechanism is related to K+ channel, inhibition of release of Ca+ stored in endoplasmic reticulum of vascular smooth muscle cells and inflow of external calcium Ca2+.
