The effects of sodium hyaluronate on mRNA expressions of matrix metalloproteinase-1, -3 and tissue inhibitor of metalloproteinase-1 in cartilage and synovium of traumatic osteoarthritis model.
- Author:
Bo QIU
1
;
Shi-qing LIU
;
Hao PENG
;
Hai-bin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anterior Cruciate Ligament; drug effects; metabolism; Cartilage, Articular; metabolism; Hyaluronic Acid; pharmacology; Matrix Metalloproteinase 3; biosynthesis; drug effects; genetics; Osteoarthritis, Knee; metabolism; pathology; RNA, Messenger; genetics; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; Synovial Membrane; metabolism; Tissue Inhibitor of Metalloproteinase-1; biosynthesis; drug effects; genetics
- From: Chinese Journal of Traumatology 2005;8(1):8-12
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe the influence of intra-articular injection of sodium hyaluronate (HA) on the mRNA expressions of matrix metalloproteinase-1,-3 (MMP-1,-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium of traumatic osteoarthritis (OA).
METHODSSixteen white rabbits underwent unilateral anterior cruciate ligament transection (ACLT) were divided into 2 groups randomly 5 weeks after transection. The experimental group rabbits received 0.3 ml of 1% HA by intra-articular injection once a week. Animals in the control group were treated under the same conditions using physiological saline. Ten weeks following surgery, cartilage and synovium were harvested. The mRNA expressions of MMP-1, MMP-3 and TIMP-1 were analyzed using reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSIn synovium, the mRNA expression of MMP-3 was suppressed in the HA injection group. HA treatment had no effect on the MMP-3 expression in cartilage. No significant difference of MMP-1 and TIMP-1 expressions in cartilage and synovium was found between the HA injection group and the control group.
CONCLUSIONSOne of the mechanisms of the therapeutic effect of HA may be the inhibition of expression of MMP-3 in synovium during early stage of traumatic OA.
