Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro.
- Author:
Li-ye YANG
1
;
Jia-kun ZHENG
;
Chao-yang WANG
;
Wen-yu LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bone Marrow Cells; cytology; metabolism; Cell Differentiation; physiology; Cell Proliferation; Humans; Immunohistochemistry; Intermediate Filament Proteins; metabolism; Mesenchymal Stromal Cells; cytology; metabolism; Nerve Tissue Proteins; metabolism; Nestin; Neurofilament Proteins; metabolism; Phosphopyruvate Hydratase; metabolism; S100 Proteins; metabolism; Schwann Cells; cytology
- From: Chinese Journal of Traumatology 2005;8(2):77-80
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the differentiative capability of adult human bone marrow mesenchymal stem cells (BMSCs) into Schwann-like cells.
METHODSBone marrows were aspirated from healthy donors and mononuclear cells were separated by Percoll lymphocytes separation liquid (1.073 g/ml) with centrifugation, cells were cultured in DMEM/F12 (1:1) medium containing 10% fetal bovine serum (FBS), 20 ng/ml epidermal growth factor (EGF) and 20 ng/ml basic fibroblast growth factor (bFGF). Cells of passage 1 were identified with immunocytochemistry.
RESULTSMononuclear cells separated by Percoll's were passaged 10 times by trypsin/ethylenediaminetetraacetic acid (EDTA) digestion in 40 days, and BMSCs increased about 6x10(7) times in this short period. Immunohistochemistry identified that BMSCs were CD34- and CD31-, but they expressed neuron specific enolase; 0.01%-0.02% of total cells expressed nestin, the marker for neural progenitor cells; 40%-50% cells stained heavily by neurofilament 200; and no glial fibrillary acidic protein (GFAP) positive cells were identified; S100 expression was detected among 0.1%-0.2% cells.
CONCLUSIONSBone marrow contains the stem cells with the ability of differentiating into Schwann-like cells, which may represent an alternative stem cell sources for neural transplantation.
