Effect of basic fibroblast growth factor on the expression of glial fibrillary acidic protein after tractive spinal cord injury in rats.
- Author:
Lei LIU
1
;
Bo LÜ
;
Chong-qi TU
;
Lei-ting CHI
;
Guang-lin WANG
;
Fu-xing PEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Evoked Potentials, Somatosensory; drug effects; Fibroblast Growth Factor 2; pharmacology; Glial Fibrillary Acidic Protein; drug effects; metabolism; Immunohistochemistry; Rats; Rats, Sprague-Dawley; Recovery of Function; Reference Values; Spinal Cord Injuries; metabolism; physiopathology; Traction
- From: Chinese Journal of Traumatology 2005;8(2):117-120
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of basic fibroblast growth factor (bFGF) on the expression of glial fibrillary acidic protein (GFAP) after tractive spinal cord injury in rats and to explore the recovery of spinal cord function.
METHODSThe rats were subjected to tractive spinal cord injury at T13-L2. Cortical somatosensory-evoked potential (CSEP) was closely monitored and when P1-N1 wave amplitude decreased to 70% of that before operation, a small-bore catheter was inserted below the injured plane through subarachnoid cavity. In the treatment groups, 20 microl of bFGF solution (containing 20 microg of bFGF) was injected through the catheter right after the operation and 1, 2, 3, 4, 8, 12 and 24 h postoperatively. In the control group, same volume of normal saline was injected and every four rats were killed at 1, 4, 7, 14 and 21 d after the operation. Combined behavior score (CBS) and electro-physiological examination were adopted to evaluate function recovery. Expression of GFAP was observed by immuno-histochemical staining and was analyzed quantitatively by computer image analysis.
RESULTSThere was statistically significant difference in GFAP-positive cells between bFGF treatment group and the control group (P<0.01). Similar tendency was indicated by the results of CBS and CSEP.
CONCLUSIONSbFGF can induce large expression of GFAP after tractive spinal cord injury in rats and promote spinal function recovery, which is highly important for spinal cord regeneration.
