The study of 3-dimensional structures of IgG with atomic force microscopy.
- Author:
Yi-gang YU
1
;
Ru-xiang XU
;
Xiao-dan JIANG
;
Yi-quan KE
Author Information
- Publication Type:Journal Article
- MeSH: Adsorption; Aluminum Silicates; Gold Colloid; Imaging, Three-Dimensional; methods; Immunoglobulin G; chemistry; Microscopy, Atomic Force; methods; Receptors, N-Methyl-D-Aspartate; chemistry
- From: Chinese Journal of Traumatology 2005;8(5):277-282
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment.
METHODSThe images and data were obtained from a contact mode and commercial Si3N4 probed tip by using atomic force microscope (AFM).
RESULTSThe anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4 A x 62.8 A x 26.1 A. On the section of the ellipse edge there were two peaks about 13 nm in width.
CONCLUSIONSUsing AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.
