Proteomics technology for screening biomarkers of oligospermic candidates.
- Author:
Huan YANG
1
;
Jie ZHANG
;
Wei ZHANG
;
Wen LONG
;
Xiao-bin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biomarkers; analysis; Humans; Male; Oligospermia; diagnosis; Protein Array Analysis; Proteins; analysis; Proteomics; methods; Semen; chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: National Journal of Andrology 2006;12(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screening specific biomarkers of oligospermic candidates from seminal plasma by proteomics technology.
METHODSThe protein spectra of 20 seminal plasma from oligospermic patients and healthy male individuals were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to compare different protein expressions.
RESULTSCompared with normal control group, a specific protein peak of molecular weight 11 022.9 in the protein spectra of oligospermic patients'semen on H4 protein-chip reduced significantly, but the protein peak of molecular weight 11 507.4 elevated apparently. For SAX-2 protein-chip, a specific protein peak of molecular weight 16 751.6 in the protein spectra of oligospermic patients' semen elevated significantly.
CONCLUSIONScreening specific proteins in seminal plasma of oligospermic patients is important for understanding the mechanism of oligospermia and screening suspected oligospermic individuals.
