Activation of Nuclear Factor Kappa B by Inducers in Gestational Tissues at Term.
- Author:
Jong Hwa KIM
1
;
Young Ah KIM
;
Soon Ha YANG
Author Information
1. Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
NF-kappa B;
Myometrium;
Amnion;
Chorion;
Cytokines;
Lipopolysacchride
- MeSH:
Amnion;
Animals;
Cesarean Section;
Chorion;
Cyclooxygenase 2;
Cytokines;
Female;
Humans;
Interleukin-1beta;
Luciferases;
Mice;
Myometrium;
NF-kappa B*;
Pregnancy;
Pregnant Women;
Prostaglandins;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Obstetrics and Gynecology
2003;46(9):1736-1740
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Nuclear factor kappa B (NF-kappa B) is a transcriptional factor in the expression of cyclooxygenase 2 (COX-2), the key enzyme in production of prostaglandins. The purpose of this study was to investigate the activation of NF-kappa B in human gestational tissues obtained from term pregnant women by various inducers. METHODS: Myometrium, chorion, and amnion were collected during cesarean section from term pregnant women not in labor. Cells from gestational tissues were isolated and cultured with Interleukin-1beta (IL-1beta), Tumor Necrosis Factor-alpha (TNF-alpha) or Lipopolysaccharide (LPS). The activation of NF-kappa B in cells of each tissues was measured by luciferase assay. RESULTS: Luciferase activity analysis showed significantly higher activity of NF-kappa B in myometrial cells treated with LPS, in chorion cells treated with IL-1beta and TNF-alpha, and in amnion cells treated with IL-1beta and LPS than control. CONCLUSION: In cells of gestational tissue at term pregnancy, the activation of NF-kappa B is cell-specific and various according to inducers.
