Curcumin-induced apoptosis in androgen-dependent prostate cancer cell line LNCaP in vitro.
- Author:
Hui GUO
1
;
Jian-Hua YU
;
Ke CHEN
;
Zhang-Qun YE
;
Guo-Chang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Curcumin; pharmacology; Dose-Response Relationship, Drug; Humans; I-kappa B Proteins; biosynthesis; Male; NF-KappaB Inhibitor alpha; Neoplasms, Hormone-Dependent; Prostatic Neoplasms; metabolism; pathology
- From: National Journal of Andrology 2006;12(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the apoptosis induction by curcumin in androgen-dependent prostate cancer cell line LNCaP).
METHODSAfter LNCaP cells were induced by 10, 25, 50, 75, 100 micromol/L curcumin respectively, the cell activity was assayed by MTT at 5, 12 and 24 hours. Flow cytometry and electronic microscopy were adopted to observe cell cycle and morphological changes of LNCaP cells at 24 hours. After 5 hours, the expression of IkappaBalpha in LNCaP cells was detected by Western blotting.
RESULTSThe growth of LNCaP cells was suppressed obviously by curcumin in dose-dependent and time-dependent manners in vitro. There were significant differences in inhibition rate among different concentrations and time groups (P < 0.05). Furthermore, curcumin could arrest the cell cycle of LNCaP cells at G2/M phase in a dose-dependent manner (P <0.01). The ratios of apoptosis were significantly higher than those of controls (P < 0. 5). Curcumin could lead to characteristic morphological changes of apoptosis in LNCaP cells after 24 hours. The expression of IkappaBalpha in LNCaP cell did not show marked changes after the exposure to different concentrations of curcumin within 5 hours.
CONCLUSIONCurcumin can suppress the growth of LNCaP, and promotes their apoptosis.
