Functional expression of adenylyl cyclase and phosphodiesterase in ejaculated human spermatozoa.
- Author:
Zhi-ming CAI
1
;
Yao-ting GUI
;
Lian-dian GUO
;
Li-bing ZHANG
;
Jian-rong ZHANG
;
He WANG
;
Jie YU
Author Information
- Publication Type:Journal Article
- MeSH: Adenylyl Cyclases; biosynthesis; Asthenozoospermia; metabolism; Cyclic AMP; metabolism; Humans; Male; Phosphoric Diester Hydrolases; biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Spermatozoa; metabolism
- From:National Journal of Andrology 2006;12(3):195-198
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the differences of expressions of adenylyl cyclase (AC) and phosphodiesterase (PDE) in ejaculated spermatozoa between healthy volunteers and the patients with asthenospermia.
METHODSEjaculated spermatozoa were collected from healthy volunteers and the patients with asthenospermia. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression of AC and PDE subtypes in human spermatozoa. The concentrations of cAMP and cGMP in the samples were detected by enzyme-linked immunosorbent assay (ELISA).
RESULTSCompared with healthy volunteers, expression of sAC mRNA and concentration of cAMP were significantly decreased in the patients with asthenospermia (P < 0.01) , while the expression of PDE4C mRNA was significantly increased at the same time (P <0.01). There were no marked differences in the expression of ACIII mRNA and concentration of cGMP between the two groups.
CONCLUSIONThe sAC down-regulation and PDE4C up-regulation are possible reasons for asthenospermia.
