Screening for Y chromosomal microdeletions in AZF region with modified multiplex PCR.
- Author:
Xiao-bin ZHU
1
;
An-liang GUO
;
Xiao-rong CAO
;
Yong LIU
;
Xu-xu SUN
;
Jian-er YAO
;
Yi WANG
;
Yi-xin WANG
;
Zheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromosome Deletion; Chromosomes, Human, Y; genetics; Genetic Loci; Humans; Male; Polymerase Chain Reaction; methods; Seminal Plasma Proteins; genetics
- From: National Journal of Andrology 2006;12(3):199-206
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEScreening for Y chromosomal microdeletions in azoospermia factor (AZF) region with modified multiplex PCR.
METHODS160 cases with spermatogenetic failure were recruited in the experimental group, while 90 cases of donors in controls. According to the laboratory guidelines supported by European Academy of Andrology (EAA) and European Molecular Genetics Quality Network (EMQN), Y chromosomal microdeletions in AZFa, b, c regions were screened with multiplex PCR. The primers of sequence targeted sites (STSs) and conditions of PCR were modified.
RESULTSUsing modified multiplex PCR, 14 (8.75%) cases with Y chromosomal microdeletions were found in the experimental group, while no case in controls. There were 12 cases in AZFc, 1 case in AZFa + b + c, 1 case in AZFb + c. According to statistics, the difference between two groups was significant (P <0.001). Reaction products could be clearly separated with agarose gel and finished in 1 h.
CONCLUSIONModified multiplex PCR protocols supported by EAA and EMQN proved to be very accurate, sensitive and quick, which could be put into screening practice for Y chromosomal microdeletions in AZF region.
