Influence of LY294002 on sperm motility in asthenozoospermia patients in vitro.
- Author:
Jun YANG
1
;
Ji-hong LIU
;
Xiao-xin GONG
;
Tao WANG
;
Jun CHEN
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Asthenozoospermia; drug therapy; Cells, Cultured; Chromones; pharmacology; Dose-Response Relationship, Drug; Humans; Male; Morpholines; pharmacology; Phosphatidylinositol 3-Kinases; antagonists & inhibitors; Sperm Motility; drug effects; Spermatozoa; drug effects
- From:National Journal of Andrology 2006;12(4):311-314
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of LY294002, a phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro, and further analyze the possible molecular mechanism.
METHODSSperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10, 30 and 60 min in all specimens by CASA.
RESULTSThe sperm in asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile cells, VSL and VAP.
CONCLUSIONLY294002 can enhance the motility of sperm in asthenozoospermia patients in vitro.
