Study of the changes of acrosomal enzyme and nitric oxide synthase, superoxide dismutase of infertile patients with positive antisperm antibody in seminal plasma.
- Author:
Hai-feng ZHANG
1
;
Jian-zhong LI
;
Chun-ying ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acrosin; metabolism; Adult; Autoantibodies; analysis; Case-Control Studies; Humans; Infertility, Male; enzymology; immunology; Male; Nitric Oxide Synthase; metabolism; Semen; enzymology; Spermatozoa; enzymology; immunology; Superoxide Dismutase; metabolism
- From: National Journal of Andrology 2006;12(4):349-351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the impacts of positive antisperm antibody (AsAb) in seminal plasma on acrosomal enzyme activity, nitric oxide synthase (NOS) and superoxide dismutase (SOD) levels of spermatozoa.
METHODSSwatch from 40 infertile patients with positive AsAb in seminal plasma as experimental group, and 40 fertile men as control group. Acrosomal enzyme activity was detected by the BAEE/ADH unitive method, NOS was detected by the redoxreaction assay, and SOD level was measured xanthine oxidase method.
RESULTSCompared with control group, acrosomal enzyme activity of spermatozoa of experimental group was significantly decreased (P <0.01), NOS activity was apparently increased (P < 0.01), and SOD level in seminal plasma was markedly decreased (P<0.01).
CONCLUSIONIt may be possible that the positive AsAb in seminal plasma beget infertility through the changes of acrosomal enzyme of spermatozoa, SOD and NOS activities in seminal plasma.
