Research of the spermatozoal gene expression with gene microarrays.
- Author:
Xiang-Ming MAO
1
;
Chun-Qiong FENG
;
Ya-Guang ZOU
;
Rong SHI
;
Yan-Bin SONG
;
Li JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation; Humans; Lymphocytes; metabolism; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; RNA; isolation & purification; Spermatozoa; metabolism; Up-Regulation
- From: National Journal of Andrology 2006;12(5):401-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo perform the detection of spermatozoal gene expression in order to accelerate the study of spermatozoal molecular biology.
METHODSTo collect the healthy adults sperm and lymphocytes respectively, and then to extract the total RNAs from them by RNeasy mini kit (QIAGEN) or Trizol reagent. Corresponding cDNAs were produced, digested, ligated, finally labeled with Cy3 (sperm) and CyS (lymphocyte) in the course of RD amplifying reactions. Hybridization with self-made microarrays contained 560 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.
RESULTSAmong the 560 probes, 72 genes were up-regulated, 321 genes were down-regulated, the others had no different expression. Furthermore, genes associated with replication, transcription, translation and regulative functions were non-different expression or down-regulated, and those belonged to the spermatogenesis associated, sperm associated antigen were up-regulated, but those involved in the glycolysis were up-regulated, in the oxidative phosphorylation were down-regulated.
CONCLUSIONIt had successfully confirmed that there were a plenty of genes expressed in sperm, furthermore the genes expressed were accorded to spermatozoal functions and characteristics.
