Nontargeted metabolomic analysis of Anoectochilus roxburghii at different cultivation stages.
10.19540/j.cnki.cjcmm.20170928.007
- Author:
Rui-Zi LI
1
;
Jun LIN
2
;
Xia-Xia WANG
2
;
Xiao-Min YU
2
;
Can-Li CHEN
3
;
Yue-Feng GUAN
2
Author Information
1. College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China.
2. Horticultural Plant Biology and Metabolomics Center, Haixia Institute of Science and Technology, Fujian Agricultural and Forestry University, Fuzhou 350002, China.
3. Fuzhou Sinao Biological Technology Co. Ltd., Fuqing 350300, China.
- Publication Type:Journal Article
- Keywords:
Anoectochilus roxburghii;
GC-TOF-MS;
UPLC-Q-TOF-MS;
metabolomics;
soil cultivation;
tissue culture
- From:
China Journal of Chinese Materia Medica
2017;42(23):4624-4630
- CountryChina
- Language:Chinese
-
Abstract:
Anoectochilus roxburghii is a traditional Chinese medicine and natural health products. In the modern cultivation system, A. roxburghii is micropropagated in tissue culture, and the plants are transferred to soil cultivation for months. However, it remains unclear about the necessity of soil cultivation for the accumulation of health beneficial compounds. In this paper, we performed nontargeted metabolomic analysis using GC-TOF-MS and UPLC-Q-TOF-MS, on A. roxburghii plants at tissue culture stage or after 3 months of soil cultivation. The results showed that the primary metabolites such as alcohols and organic acids are abundant in the tissue culture plants. In contrast, polysaccharide, nucleoside, esters and secondary metabolites such as flavonoids, terpenoids were significantly accumulated in cultivated seedlings. Flavonoids and polysaccharides are considered as the principle effective components in A. roxburghii. Soil cultivation period is therefore essential for the accumulation of these metabolites.
