Study on early screening of high saponin ginseng lines.
- Author:
Li-Shan HAO
1
;
Yu-Gang GAO
1
;
Pu ZANG
1
;
Yan ZHAO
1
;
Zhong-Mei HE
1
;
Hong-Yan ZHU
1
;
He YANG
1
;
Shang-Li LIU
1
;
Jing-Ting LIU
1
;
Lian-Xue ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: PCR; ginseng breeding; primer screening; system optimization
- From: China Journal of Chinese Materia Medica 2017;42(24):4775-4781
- CountryChina
- Language:Chinese
- Abstract: The study aims at screening the specific bands by PCR, quickly and accurately evaluating the quality of ginseng seeding, accelerating the process of ginseng breeding. Based on the correlation of genetic differences and saponin content between individuals, a pair of specific primer GC1 was screened by PCR. According to the experiment by L16 (45) orthogonal test, a PCR system most suitable for GC1 was established, which came out total 25 μL reaction system containing DNA 2.60 mg•L⁻¹, Mg²⁺ 1.44 mmol•L⁻¹, dNTP 0.19 mmol•L⁻¹, primer 0.32 μmol•L⁻¹ and Taq enzyme concentration 0.076 U•μL⁻¹. By comparing the saponin content and the GC1 PCR electrophoretogram of samples, the ginseng, with 1 200 bp specific band by PCR of GC1, the contents of 9 monosodium saponins and their additions were higher than others, which provided a reliable method for accelerating the process of ginseng breeding. The sequence was sequenced and 99% homologous to glycerol-3-phosphate dehydrogenase.
